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AM12014PU-S DLG4 / PSD95 antibody

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Mouse anti Bovine, Human, Mouse, Rat DLG4 / PSD95 7E3

AM12014PU-S

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  • AM12014PU-S
  • AM12014PU-S

Product Description for DLG4 / PSD95

Mouse anti Bovine, Human, Mouse, Rat DLG4 / PSD95 7E3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for DLG4 / PSD95

Product Category Primary Antibodies
Target Category
Quantity 25 µg
Synonyms DLGH4, Disks large homolog 4, PSD-95, Postsynaptic density protein 95, SAP-90, SAP90, Synapse-associated protein 90
Presentation Purified
Reactivity Bov, Hu, Ms, Rt
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone 7E3
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P31016
Immunogen:
Recombinant rat PSD-95
GeneID:
29495
Isotype control AM03096PU-N
Application Immunflourescence.
Immunoohistochemistry.
Western blot:
1 µg/ml was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract.
Background Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an Nterminal variable segment followed by three aminoterminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain.
The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4).
PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).
Concentration 1.0 mg/ml
General Readings
  1. Chetkovich D.M., Bunn R.C., Kuo S.H., Kawasaki Y., Kohwi M., and Bredt D.S. (2002) J Neurosci. 22(15): 6415-25.
  2. Cao J., Viholainen J.I., Dart C., Warwick H.K., Levland M.L. and Courtney M.J. (2005) J Cell Biol. 168(1): 117-26.
  3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268.
  4. Irie M. et al. (1997) Science 277(5331): 1511-5.
  5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73.
  6. Yao W.D. et al. (2004) Neuron 41: 625-38.
  7. Cline H. (2005) Curr Biol. 15: R203-5.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography.
Buffer System:
PBS pH 7.4 containing 0.09% Sodium Azide as preservative in 50% Glycerol.
State:
Liquid purified Ig fraction.
Purified
Species Reactivity
Species reactivity (tested):
Mouse, Rat, Bovine, Human.
Specificity
Specificity:
This antibody detects a ~100kDa protein corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots.
Additional cross-reactive bands are detected at ~80 kDa and 50 kDa in Rat and Mouse samples.

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