discontinued

SM5003P DLG4 / PSD95 antibody

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Mouse anti Human, Mouse, Rat, Xenopus DLG4 / PSD95 7E3-1B8

Product Description for DLG4 / PSD95

Mouse anti Human, Mouse, Rat, Xenopus DLG4 / PSD95 7E3-1B8.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for DLG4 / PSD95

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms DLGH4, Disks large homolog 4, PSD-95, Postsynaptic density protein 95, SAP-90, SAP90, Synapse-associated protein 90
Presentation Purified
Reactivity Hu, Ms, Rt, Xen
Applications C, F, ICC/IF, IP, WB
Clonality Monoclonal
Clone 7E3-1B8
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q62108
Immunogen:
Purified recombinant rat PSD-95.
GeneID:
13385
Isotype control AM03096PU-N
Application Immunoprecipitation.
Immunocytochemistry (1/200).
Immunofluorescence (1/200): Staining of PSD-95 in rat hippocampal cells with this antibody yields a staining pattern coincident with NMDA receptor; fixation with cold methanol is recommended.
Western Blot (1/2000): Detects an ~95 kDa protein representing PSD-95 from rat brain extracts.
Flow Cytometry (2 µg/test).
Immunohistochemistry on frozen sections.
Background Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain.
PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
Concentration 1.0 mg/ml
General Readings
  1. Gomperts SN. Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell. 1996 Mar 8;84(5):659-62. PubMed PMID: 8625403.
  2. Stöhr H, Stojic J, Weber BH. Cellular localization of the MPP4 protein in the mammalian retina. Invest Ophthalmol Vis Sci. 2003 Dec;44(12):5067-74. PubMed PMID: 14638699.
  3. Colledge M, Snyder EM, Crozier RA, Soderling JA, Jin Y, Langeberg LK, et al. Ubiquitination regulates PSD-95 degradation and AMPA receptor surface expression. Neuron. 2003 Oct 30;40(3):595-607. PubMed PMID: 14642282. (Free PMC Article available, 8 images available)
  4. Cuadra AE, Kuo SH, Kawasaki Y, Bredt DS, Chetkovich DM. AMPA receptor synaptic targeting regulated by stargazin interactions with the Golgi-resident PDZ protein nPIST. J Neurosci. 2004 Aug 25;24(34):7491-502. PubMed PMID: 15329396.
  5. Takahashi H, Sekino Y, Tanaka S, Mizui T, Kishi S, Shirao T. Drebrin-dependent actin clustering in dendritic filopodia governs synaptic targeting of postsynaptic density-95 and dendritic spine morphogenesis. J Neurosci. 2003 Jul 23;23(16):6586-95. PubMed PMID: 12878700.
  6. Prange O, Wong TP, Gerrow K, Wang YT, El-Husseini A. A balance between excitatory and inhibitory synapses is controlled by PSD-95 and neuroligin. Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13915-20. Epub 2004 Sep 9. PubMed PMID: 15358863. (Free PMC Article available, 6 images available)
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS buffer with 0.05% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody detects Post Synaptic Density 95 kDa (PSD-95).
Species:
Human, Xenopus, Mouse and Rat.
Gene ID 13385

Accessory Products

Proteins and/or Positive Controls

Positive controls for DLG4 / PSD95 (1 products)

Catalog No. Species Pres. Purity   Source  

DLG4 293T Cell Transient Overexpression Lysate(Denatured)

DLG4 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-DLG4 antibody (H00001742-B01) by Western Blots.
  Abnova Taiwan Corp.
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