TA305925 DNA ligase 1 antibody

Goat Anti-LIG1 / DNA ligase I Antibody

See related secondary antibodies

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0.1 mg / €325.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human, Rat DNA ligase 1

Product Description for DNA ligase 1

Goat anti Human, Rat DNA ligase 1.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for DNA ligase 1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1
Presentation Aff - Purified
Reactivity Hu, Rt
Applications WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P18858
Immunogen:
Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1
AA Sequence:
C-RVREDKQPEQATTS
GeneID:
3978
Application Peptide ELISA: 1:16000 (limit dilution).
Western blot: 0.1-0.3µg/ml. Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Background Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.
Concentration 0.5 mg/ml
General Readings Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A: Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites. J. Biol. Chem. 2003 Sep 278 (39): 37761-7. PMID: 12851383
Storage Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Buffer System:
Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Rat
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects DNA ligase 1 (Internal).

Accessory Products

Proteins and/or Positive Controls

Proteins for DNA ligase 1 (3 products)

Catalog No. Species Pres. Purity   Source  

DNA ligase 1

DNA ligase 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

DNA ligase 1

DNA ligase 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

DNA ligase 1

DNA ligase 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for DNA ligase 1 (1 products)

Catalog No. Species Pres. Purity   Source  

LIG1 overexpression lysate

LIG1 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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