AM05299PU-N DNA Ligase I antibody

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Mouse anti Bovine, Human DNA Ligase I 1A9

Product Description for DNA Ligase I

Mouse anti Bovine, Human DNA Ligase I 1A9.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for DNA Ligase I

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Bov, Hu
Applications IP, WB
Clonality Monoclonal
Clone 1A9
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized with bovine DNA ligase I protein and myeloma Sp2/0 cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA.
Western Blot (1-5 µg/ml).
Positive Control HeLa or DiFi cells.
Background Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae CDC9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Concentration Lot specific
General Readings
  1. Prigent C, Satoh MS, Daly G, Barnes DE, Lindahl T. Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I. Mol Cell Biol. 1994 Jan;14(1):310-7. PubMed PMID: 8264597. (Free PMC Article available)
  2. Mackenney VJ, Barnes DE, Lindahl T. Specific function of DNA ligase I in simian virus 40 DNA replication by human cell-free extracts is mediated by the amino-terminal non-catalytic domain. J Biol Chem. 1997 Apr 25;272(17):11550-6. PubMed PMID: 9111070.
  3. Bentley D, Selfridge J, Millar JK, Samuel K, Hole N, Ansell JD, et al. DNA ligase I is required for fetal liver erythropoiesis but is not essential for mammalian cell viability. Nat Genet. 1996 Aug;13(4):489-91. PubMed PMID: 8696349.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Buffer System:
PBS containing 0.08% Sodium Azide as preservative.
Liquid purified IgG fraction.
Recognizes a protein of ~125kDa, identified as the DNA Ligase I.
Human and Bovine.

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