DM3068 DNA Polymerase beta antibody

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Mouse anti Amphibian, Bovine, Hamster, Human, Mouse, Rat DNA Polymerase beta 18S


Product Description for DNA Polymerase beta

Mouse anti Amphibian, Bovine, Hamster, Human, Mouse, Rat DNA Polymerase beta 18S.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for DNA Polymerase beta

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3181
Presentation Purified
Reactivity Amph, Bov, Hst, Hu, Ms, Rt
Applications IP, WB
Clonality Monoclonal
Clone 18S
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Rat DNA polymerase beta protein

Format: This antibody is supplied as liquid, Protein G affinity purified immunoglobulin fraction in 10 mM PBS, pH 7.4, with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Effect on Polymerase Activity (Non-inhibitory)(1).
Immunoprecipitation (Denatured verified); Use Protein G; Ab at 2µg/mg protein lysate.
Western Blotting: Ab 1-2µg/ml for 2hrs at RT. Recommended positive control: HeLa Cells. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Srivastava DK, et. al. Journ
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
DNA polymerase b comprises an amino-terminal 8 kD domain and a carboxyterminal 31 kD domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA Polymerase b, a DNA repair polymerase, is constituitively expressed in cultured cells. Reacts with Human, Cow, Mouse, Rat, Hamster, and Xenopus. Others not tested. Cellular Localization: nuclear.

Mol. Wt. of Antigen: 39kD

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