TA305748 DNA polymerase lambda (C-term) antibody

Goat Anti-POLL / BETA-N Antibody

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human, Mouse DNA polymerase lambda

Product Description for DNA polymerase lambda

Goat anti Human, Mouse DNA polymerase lambda.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for DNA polymerase lambda

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda
Presentation Aff - Purified
Reactivity Hu, Ms
Applications P, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UGP5
Immunogen:
Peptide from C Terminus of the protein sequence according to NP_037406.1
AA Sequence:
C-LGLPYREPAERDW
GeneID:
27343
Property C-term
Add. information It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?
Application Peptide ELISA: Limit dilution 1:32000.
Western blot: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1 µg/ml.
Immunohistochemistry: 5-10 µg/ml. In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria.
Background DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.
Concentration 0.5 mg/ml
General Readings Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L.
DNA polymerase lambda, a novel DNA repair enzyme in human cells.
J Biol Chem. 2002 Apr 12;277(15):13184-91.
PMID: 11821417
Format
Purification:
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Buffer System:
Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects DNA Polymerase Lambda at C-term.

Accessory Products

Proteins and/or Positive Controls

Proteins for DNA polymerase lambda (4 products)

Catalog No. Species Pres. Purity   Source  

DNA polymerase lambda

DNA polymerase lambda Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DNA polymerase lambda

DNA polymerase lambda Human
  Abnova Taiwan Corp.

DNA polymerase lambda

DNA polymerase lambda Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

DNA polymerase lambda

DNA polymerase lambda Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for DNA polymerase lambda (2 products)

Catalog No. Species Pres. Purity   Source  

DNA Polymerase lambda Lysate

Western Blot: DNA Polymerase lambda Lysate [NBL1-14573] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for POLL
  Novus Biologicals Inc.

POLL overexpression lysate

POLL overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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