AM12027PU-N DNA/RNA Damage antibody

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0.1 mg / €360.00

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Mouse anti DNA/RNA Damage 15A3


Product Description for DNA/RNA Damage

Mouse anti DNA/RNA Damage 15A3.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay.

Properties for DNA/RNA Damage

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine
Presentation Purified
Applications C, E
Clonality Monoclonal
Clone 15A3
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

8-hydroxy-guanosine-BSA and – casein conjugates
Application ELISA.
Immunohistochemistry on frozen sections.
Background DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1).
8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-ydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
Concentration 0.65 mg/ml
General Readings
  1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241.
  2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15.
  3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432.
  4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283.
  5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9.
  6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9.
  7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
PBS, in 50 % glycerol, 0.09 % sodium azide
Liquid Ig fraction
This antibody detects markers of oxidative damage to DNA (8-
hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

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