TA336580 DNMT3A antibody

Mouse Monoclonal DNMT3A Antibody (64B1446)

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0.1 mg / €474.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Mouse DNMT3A 64B1446

TA336580

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Product Description for DNMT3A

Mouse anti Human, Mouse DNMT3A 64B1446.
Presentation: Purified
Product is tested for ChIP assay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for DNMT3A

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DNA (cytosine-5)-methyltransferase 3A, DNA MTase HsaIIIA, DNA methyltransferase HsaIIIA, M.HsaIIIA
Presentation Purified
Reactivity Hu, Ms
Applications ChIP, F, ICC/IF, P, WB
Clonality Monoclonal
Clone 64B1446
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y6K1
Immunogen:
This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
GeneID:
1788
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application WB: 2 ug/ml
ChIP:
FC: 1 ug per million cells
IF: 1:10-1:500
IHC-P: 5 ug/ml
Background Methylation of D at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic D may be linked with development of cancer. To date, 3 families of mammalian D methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and ictivation of this gene causes global demethylation of genomic D and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its ictivation does not affect D methylation or maintence of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that turally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Format
Purification:
Protein G purified
Buffer System:
PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
State:
Purified

Accessory Products

Proteins and/or Positive Controls

Proteins for DNMT3A (6 products)

Catalog No. Species Pres. Purity   Source  

DNMT3A (transcript variant 3)

DNMT3A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DNMT3A

DNMT3A Human Purified
  Abnova Taiwan Corp.

DNMT3A

DNMT3A Human Purified
  Abnova Taiwan Corp.

DNMT3A

DNMT3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

DNMT3A

DNMT3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

DNMT3A

DNMT3A Human
  Abnova Taiwan Corp.

Positive controls for DNMT3A (5 products)

Catalog No. Species Pres. Purity   Source  

DNMT3A 293T Cell Transient Overexpression Lysate(Denatured)

DNMT3A 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Dnmt3a Lysate

Western Blot: Dnmt3a Lysate [NBL1-09970] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for DNMT3A
  Novus Biologicals Inc.

DNMT3A overexpression lysate

DNMT3A overexpression lysate
  OriGene Technologies, Inc.

DNMT3A overexpression lysate

DNMT3A overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

DNMT3A overexpression lysate

DNMT3A overexpression lysate
  OriGene Technologies, Inc.
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