Product Description for DNMT3A
Mouse anti Human, Mouse DNMT3A 64B1446.
Presentation: Purified
Product is tested for ChIP assay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Presentation: Purified
Product is tested for ChIP assay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Properties for DNMT3A
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 mg |
| Synonyms | DNA (cytosine-5)-methyltransferase 3A, DNA MTase HsaIIIA, DNA methyltransferase HsaIIIA, M.HsaIIIA |
| Presentation | Purified |
| Reactivity | Hu, Ms |
| Applications | ChIP, F, ICC/IF, P, WB |
| Clonality | Monoclonal |
| Clone | 64B1446 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | WB: 2 ug/ml ChIP: FC: 1 ug per million cells IF: 1:10-1:500 IHC-P: 5 ug/ml |
| Background | Methylation of D at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic D may be linked with development of cancer. To date, 3 families of mammalian D methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and ictivation of this gene causes global demethylation of genomic D and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its ictivation does not affect D methylation or maintence of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that turally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
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Accessory Products
Proteins and/or Positive Controls
Proteins for DNMT3A (6 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP313064 |
DNMT3A (transcript variant 3) |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
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| OriGene Technologies, Inc. | |||||||
| H00001788-P01 |
DNMT3A |
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|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00001788-P01-25 |
DNMT3A |
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|
|
Human | Purified | |||||
| Abnova Taiwan Corp. | |||||||
| H00001788-Q01-10 |
DNMT3A |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001788-Q01-25 |
DNMT3A |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P0160 |
DNMT3A |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for DNMT3A (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00001788-T01 |
DNMT3A 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| NBL1-09970 |
Dnmt3a Lysate |
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![Western Blot: Dnmt3a Lysate [NBL1-09970] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for DNMT3A](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Dnmt3a%20Lysate-Western%20Blot-NBL1-09970-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| LY406275 |
DNMT3A overexpression lysate |
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|
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| OriGene Technologies, Inc. | |||||||
| LY411621 |
DNMT3A overexpression lysate |
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|
0.1 mg /
€495.00
|
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| OriGene Technologies, Inc. | |||||||
| LY430430 |
DNMT3A overexpression lysate |
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|
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| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for DNMT3A (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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