AP02521PU-N DOK2 / p56 dok2 pTyr299 antibody

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0.1 mg / €320.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human DOK2 / p56 dok2

AP02521PU-N

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Product Description for DOK2 / p56 dok2

Rabbit anti Human DOK2 / p56 dok2.
Properties: pTyr299
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for DOK2 / p56 dok2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), p56Dok-2
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O60496
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).
GeneID:
9046
Property pTyr299
Application Western Blot: 1/500-1/1000.
Immunofluorescence: 1/100-1/200.
Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Background Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. DOK2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. DOK2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the DOK1 and DOK2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the DOK proteins serve as mediators of bcr-abl signaling. The role of DOK proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9;22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.
Concentration 1.0 mg/ml
General Readings
  1. Feng Cong, et,al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325.
  2. Serge Lemay, et,al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754.
  3. Ute Schaeper, et,al.(2000) J. Cell Biol ; 149: 1419.
  4. Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of p56Dok-2 only when phosphorylated
at Tyrosine 299.
Species:
Human.
Gene ID 9046

Accessory Products

Proteins and/or Positive Controls

Proteins for DOK2 / p56 dok2 (3 products)

Catalog No. Species Pres. Purity   Source  

DOK2 / p56 dok2

DOK2 / p56 dok2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

DOK2 / p56 dok2

DOK2 / p56 dok2 Human in vitro transl.
  Abnova Taiwan Corp.

DOK2 / p56 dok2

DOK2 / p56 dok2 Human
  Abnova Taiwan Corp.

Positive controls for DOK2 / p56 dok2 (2 products)

Catalog No. Species Pres. Purity   Source  

DOK2 Lysate

Western Blot: DOK2 Lysate [NBL1-09978] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for DOK2
  Novus Biologicals Inc.

DOK2 overexpression lysate

DOK2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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