AM08434PU-N DYKDDDDK Epitope Tag antibody

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0.1 mg / €310.00
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Mouse anti DYKDDDDK Epitope Tag 29E4.G7.H11

AM08434PU-N

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  • AM08434PU-N

Product Description for DYKDDDDK Epitope Tag

Mouse anti DYKDDDDK Epitope Tag 29E4.G7.H11.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Purified
Applications E, F, IP, WB
Clonality Monoclonal
Clone 29E4.G7.H11
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Produced in Mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
Remarks:
Residues of Glycine and Cysteine were added to the termini to facilitate coupling.
Application This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope.
The antibody recognizes the epitope tag fused to either the amino- or carboxy-termini of targeted proteins.
The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7.
DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/20,000.
Flow Cytometry: 1/2,000-1/10,000.
Background Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques. 1996 Jan;20(1):136-41. PubMed PMID: 8770418.
  2. Slootstra JW, Kuperus D, Plückthun A, Meloen RH. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. Mol Divers. 1997;2(3):156-64. PubMed PMID: 9238646.
  3. Robeva AS, Woodard R, Luthin DR, Taylor HE, Linden J. Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure. Biochem Pharmacol. 1996 Feb 23;51(4):545-55. PubMed PMID: 8619901.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography followed by extensive dialysis against the buffer.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtered) purified IgG fraction.
Purified
Specificity
Specificity:
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins.
This antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins.
This monoclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG™ epitope tag.
In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

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