AM20780PU-L DYKDDDDK Epitope Tag antibody

See related secondary antibodies

Search for all "DYKDDDDK Epitope Tag"

Quick Overview

Mouse anti All Species DYKDDDDK Epitope Tag 3B9


More Views

  • AM20780PU-L

Product Description for DYKDDDDK Epitope Tag

Mouse anti All Species DYKDDDDK Epitope Tag 3B9.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Aff - Purified
Reactivity All Species
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone 3B9
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
Application ELISA: 1/2000.
Western blot: 1/5000, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1 TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.
Immunofluorescence: 1/1000.
Immunoprecipitation: 1/100.
Background Epitope tags are useful for the labeling and detection of proteins using Immunoblotting, Immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Concentration 1 mg/ml
General Readings
  1. Brizzard BL, Chubet RG, Vizard DL. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 1994 Apr;16(4):730-5. PubMed PMID: 8024796.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS, pH 7.2
0.05% Sodium Azide
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat.
This antibody detects exogenously expressed DYKDDDDK proteins in cells.
Use an anti-Mouse secondary antibody to detect the 3B9 antibody

Accessory Products

  • LinkedIn