AP05574PU-N DYKDDDDK Epitope Tag antibody

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0.1 mg / €530.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti DYKDDDDK Epitope Tag

AP05574PU-N

Product Description for DYKDDDDK Epitope Tag

Rabbit anti DYKDDDDK Epitope Tag.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Purified
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Application ELISA: 1:9000 - 1:450000.
Western Blot: 1:2000 - 1:10000.
Caution (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Concentration 0.5 mg/ml
General Readings
  1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204 - 1210.
  2. Slootstra JW, Kuperus D, Plückthun A, Meloen RH. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. Mol Divers. 1997;2(3):156-64. PubMed PMID: 9238646.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.02M Potassium phosphate with 0.15M Sodium chloride pH7.2 containing 0.09% Sodium Azide (NaN3)
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This antibody is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. This antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.
In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.

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