AP08111PU-N DYKDDDDK Epitope Tag antibody

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0.1 mg / €320.00
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Quick Overview

Rabbit anti Mammalia DYKDDDDK Epitope Tag

Product Description for DYKDDDDK Epitope Tag

Rabbit anti Mammalia DYKDDDDK Epitope Tag.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Aff - Purified
Reactivity All, Mam
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

The antiserum was produced by immunizing Rabbit with synthesized peptide containing the Flag epitope (D-Y-K-D-D-D-D-K).
Application Western Blot (1/500-1/1000).
Background Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
General Readings
  1. Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533.
  2. Einhauer A, et al. (2001) J Biochem Biophys Methods; 49(1-3):455-465.
  3. Brizzard, B.L. et al. (1994) Biotechniques; 16:730-735.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Immunoaffinity Chromatography using epitope-specific peptide.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Liquid purified Ig fraction.
Aff - Purified
Flag-Tag Polyclonal Antibody AP08111PU detects Flag-tagged proteins overexpressed in cells.
The antibody recognizes the Flag-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.

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