DP3002 DYKDDDDK Epitope Tag antibody

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Rabbit anti DYKDDDDK Epitope Tag


Product Description for DYKDDDDK Epitope Tag

Rabbit anti DYKDDDDK Epitope Tag.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Purified
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Amino acid sequence: CDYKDDDDK-GC~KLH

Format: This antibody is supplied as liquid, purified immunoglobulin fraction in
10 mM phosphate, 150 mM NaCl, pH 7.4,and 0.05% sodium azide
Application This antibody recognizes the DYKDDDDK epitope located on DYKDDDDK-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. Western Blot: 10-20 µg/ml using E. coli periplasmic extract expressing
N-terminal DYKDDDDK-fusion protein (2Hrs RT).
Indirect Immunofluorescence: 10-20 µg/ml using 293T cells transfected with a plasmid encoding DYKDDDDK-JNK.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration total immunoglobulin 3.3 mg/ml
Storage Store the antibody at 4°C. Do not freeze! Shelf life: on
The DYKDDDDK epitope is an expression tag commonly used in protein expression expts. When compared to its corresponding native protein, a DYKDDDDK-tagged protein migrates at a larger size on SDS-PAGE, which is equivalent to the size of the epitope tag. The size of the DYKDDDDK~ epitope may vary depending on the expression vector being utilized, but usually range fom 3-5 kD.

Mol. WT. of Antigen: DYKDDDDK-tag, 3-5 kD

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