NB100-63146 DYKDDDDK Epitope Tag antibody

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0.1 mg / €640.00
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Quick Overview

Rabbit anti DYKDDDDK Epitope Tag


Product Description for DYKDDDDK Epitope Tag

Rabbit anti DYKDDDDK Epitope Tag.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Aff - Purified
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Application ELISA, Western Blot, This antibody is useful for ELISA, Western Blot In Western blotting of bacterial extracts, this antibody has been shown not to cross-react with any endogenous proteins., ELISA Western Blot
Background This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.
Concentration 0.5 mg/ml
General Readings
  1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210.
  2. Slootstra JW, Kuperus D, Plückthun A, Meloen RH. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5. Mol Divers. 1997;2(3):156-64. PubMed PMID: 9238646.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG purified
Buffer System:
0.09% Sodium Azide
IgG purified
Aff - Purified
NB100-63146 is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. This antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Cross reacts with synthetic peptide. Not yet tested in other species.

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