discontinued

SP5388P DYKDDDDK Epitope Tag antibody

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Rabbit anti DYKDDDDK Epitope Tag

Product Description for DYKDDDDK Epitope Tag

Rabbit anti DYKDDDDK Epitope Tag.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for DYKDDDDK Epitope Tag

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms D-tag, ECS Epitope Tag, ECS-tag, FLAG Epitope Tag, FLAG-tag
Presentation Aff - Purified
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide: DYKDDDDK epitope tag sequence.
Application Western blot: 1/500.
Immunofluorescence: 1.0 µg/ml.
Background Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), GFP, maltose binding protein (MBP) etc.
Concentration 1.0 mg/ml
General Readings 1.JBC Jun 2005 Manuscript M504087200
Am J Physiol Heart Circ Physiol 287, H652-H658, 2004
2. Mol. Biol. Cell, Vol 14: 3675-3689, Sept 2003
3. J. Biol. Chem., Vol 278: 30788 - 30795, Aug 2003
4. J. Virol., Vol 77: 10584 - 10593, Oct 2003
5. J. Biol. Chem., Vol 278: 41198 - 41204, Oct 2003
6. Mol. Cell. Biol. Vol. 25, No. 10 4046-4061 May 2005
7. JBC 277(23):20702-20710, 2002.
8. JBC 277(23):20750-20755, 2002.
9. J. Cell Science 117, 723-734 Feb 2004, doi: 10.1242/jcs.00878 />10. JBC Vol 279, No 12, 47732-47739, Nov 2004
11. JBC 2005, manuscript number M503770200.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Epitope Affinity Chromatography.
Buffer System:
PBS containing 1 mg/ml BSA as stabilizer and 0.05% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Aff - Purified
Specificity
Specificity:
This antibody is useful for detecting the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.

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