AP51398PU-N EIF3K / EIF3S12 (C-term) antibody

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0.4 ml / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human EIF3K / EIF3S12

Product Description for EIF3K / EIF3S12

Rabbit anti Human EIF3K / EIF3S12.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for EIF3K / EIF3S12

Product Category Primary Antibodies
Target Category
Quantity 0.4 ml
Synonyms Eukaryotic translation initiation factor 3 subunit 12, Eukaryotic translation initiation factor 3 subunit K, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Molecular weight 25060 Da
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UBQ5
Immunogen:
KLH conjugated synthetic peptide between 175-204 amino acids from the C-terminal region of human EIF3K
GeneID:
27335
Property C-term
Application ELISA: 1/1000.
Western Blot: 1/100 - 1/500.
Background The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).
Concentration lot specific
Product Citations
Purchased from Acris:
  1. Zhou M, Sandercock AM, Fraser CS, Ridlova G, Stephens E, Schenauer MR, et al. Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18139-44. doi: 10.1073/pnas.0801313105. Epub 2008 Jul 1. PubMed PMID: 18599441. (Free PMC Article available, 5 images available)
  2. Lin YM, Chen YR, Lin JR, Wang WJ, Inoko A, Inagaki M, et al. eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions. J Cell Sci. 2008 Jul 15;121(Pt 14):2382-93. doi: 10.1242/jcs.021394. Epub 2008 Jun 24. PubMed PMID: 18577580.
  3. Masutani M, Sonenberg N, Yokoyama S, Imataka H. Reconstitution reveals the functional core of mammalian eIF3. EMBO J. 2007 Jul 25;26(14):3373-83. Epub 2007 Jun 21. PubMed PMID: 17581632. (Free PMC Article available, 7 images available)
  4. Damoc E, Fraser CS, Zhou M, Videler H, Mayeur GL, Hershey JW, et al. Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry. Mol Cell Proteomics. 2007 Jul;6(7):1135-46. Epub 2007 Feb 23. PubMed PMID: 17322308.
  5. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, et al. Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Biol. 2007;3:89. Epub 2007 Mar 13. PubMed PMID: 17353931. (Free PMC Article available, 9 images available)
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity chromatography on Protein A
Buffer System:
PBS
Preservatives:
0.09% (W/V) sodium azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody reacts to EIF3K.
Gene ID 27335

Accessory Products

Proteins and/or Positive Controls

Proteins for EIF3K / EIF3S12 (8 products)

Catalog No. Species Pres. Purity   Source  

EIF3K / EIF3S12

EIF3K / EIF3S12 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

EIF3K / EIF3S12 (1-218, His-tag)

EIF3K / EIF3S12 Human Purified > 95 % E. coli
0.25 mg / €750.00
  OriGene Technologies GmbH

EIF3K / EIF3S12 (1-218, His-tag)

EIF3K / EIF3S12 Human Purified > 95 % E. coli
50 µg / €300.00
  OriGene Technologies GmbH

EIF3K / EIF3S12

EIF3K / EIF3S12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

EIF3K / EIF3S12

EIF3K / EIF3S12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

EIF3K / EIF3S12 (50-94, His-tagged)

EIF3K / EIF3S12 Human Purified > 90 % E. coli
  Genesis Biotech Inc.

EIF3K / EIF3S12

EIF3K / EIF3S12 Human Purified
  Novus Biologicals Inc.

EIF3K / EIF3S12

EIF3K / EIF3S12 Human Purified
  Novus Biologicals Inc.

Positive controls for EIF3K / EIF3S12 (1 products)

Catalog No. Species Pres. Purity   Source  

EIF3K overexpression lysate

EIF3K overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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