BP429 Elastase (Neutrophil) antibody

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Rabbit anti Human Elastase (Neutrophil)


Product Description for Elastase (Neutrophil)

Rabbit anti Human Elastase (Neutrophil).
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for Elastase (Neutrophil)

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu
Applications E
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified human Neutrophil Elastase.
Application ELISA (1/700-1/2800): In a simple ELISA with 5 µg/ml of antigen in the coating buffer, and an HRP conjugated secondary antibody to detect, a 1/1400 dilution of BP429 gave an absorbance of 1.0 at 405 nm.
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A
Concentration 3.6 mg/ml (OD280)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Ammonium Sulphate Fractionation.
Buffer System:
PBS, pH 7.2 containing 0.09% Sodium Azide as preservative.
Liquid purified Ig fraction.
This antibody is specific for Neutrophil Elastase.

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