discontinued

DM3153 Elastase (neutrophil) antibody

See related secondary antibodies

Search for all "Elastase (neutrophil)"

0.5 ml / €250.00

Quick Overview

Mouse anti Human Elastase (neutrophil) NP57

DM3153

Product Description for Elastase (neutrophil)

Mouse anti Human Elastase (neutrophil) NP57.
Presentation: Supernatant
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Elastase (neutrophil)

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone NP57
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Human Neutrophil Granule Proteins.
Application Immunohistochemistry on frozen and paraffin embedded sections (1:50 to 1:150; Prolonged fixation in buffered formalin can destroy the Epitope). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.15-0.18 mg/ml
General Readings
  1. Pulford KA, Erber WN, Crick JA, Olsson I, Micklem KJ, Gatter KC, et al. Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. J Clin Pathol. 1988 Aug;41(8):853-60. PubMed PMID: 2844860. (Free PMC Article available)
  2. Kramps JA, van der Valk P, van der Sandt MM, Lindeman J, Meijer CJ. Elastase as a marker for neutrophilic myeloid cells. J Histochem Cytochem. 1984 Apr;32(4):389-94. PubMed PMID: 6561228.
  3. Briggs RC, Glass WF, Monteil MM, Hnilica LS. Lactoferrin: nuclear localization in the human neutrophilic gran
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Supernatant
Specificity
Specificity:
This antibody reacts with human neutrophil elastase, a neutral protease which plays a role in microbial killing and in initiating tissue injury. This antibody recognizes neutrophils and their precursors. This antigen is sharply localized in individual myeloid cells. Cellular Localization: cytoplasmic.

MW of Antigen: 30kD

Aliquot. Instruct.: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

Accessory Products

  • LinkedIn