BP430 Elastase (Pankreatic) antibody

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Sheep anti Human, Porcine Elastase (Pankreatic)


Product Description for Elastase (Pankreatic)

Sheep anti Human, Porcine Elastase (Pankreatic).
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for Elastase (Pankreatic)

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu, Por
Applications E
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Highly purified porcine pancreatic Elastase.
Application ELISA (1/3000-1/12000): In a simple screening ELISA using 1 μg/well of porcine elastase the 50% maximal OD is obtained at a dilution of around 1/6000, with alkaline phosphatase conjugated anti-sheep for detection. All incubations at 18°C using pNPP substrate, with OD measurements at 405 nm. The end point titre for this antibody is 1/80,000.
Also reactive with human neutrophil elastase in simple competitive assay at similar dilutions (1/5,000) using plates coated with porcine antigen, giving a sensitive assay for this analyte.
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.
Concentration 5.7 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Chromatography on Protein G
Buffer System:
PBS, pH 7.2 without preservatives.
Liquid purified Ig fraction.
Reacts with Porcine Elastase and Human neutrophil elastase.
This antibody is expected to react with elastase from other species.
Human and Pig.

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