AM32176PU-N Endothelial Cell Internalizing Surface Antigen antibody

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1 ml / €350.00
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Quick Overview

Mouse anti Human Endothelial Cell Internalizing Surface Antigen GES-HB

AM32176PU-N

Product Description for Endothelial Cell Internalizing Surface Antigen

Mouse anti Human Endothelial Cell Internalizing Surface Antigen GES-HB.
Presentation: Purified
Product is tested for Frozen Sections.

Properties for Endothelial Cell Internalizing Surface Antigen

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu
Applications C
Clonality Monoclonal
Clone GES-HB
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Flow Cytometry.
Immunohistochemistry on frozen sections: 1/20; Fixation with 1% paraformaldehyde for 5 minutes is recommended for fluorescence staining of endothelial cells.
Caution

Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.

Concentration 0,2 mg/ml (after reconstituion)
General Readings
  1. Trubetskaya OV, Trubetskoy VS, Domogatsky SP, Rudin AV, Popov NV, Danilov SM, et al. Monoclonal antibody to human endothelial cell surface internalization and liposome delivery in cell culture. FEBS Lett. 1988 Feb 8;228(1):131-4. PubMed PMID: 3342871.
  2. Muzykantov VR, Trubetskaya OV, Puchnina EA, Sakharov DV, Domogatsky SP. Cytotoxicity of glucose oxidase conjugated with antibodies to target cells: killing efficiency depends on the conjugate internalization. Biochim Biophys Acta. 1990 Jun 12;1053(1):27-31. PubMed PMID: 2364115.
Format
Purification:
PEG6000 precipitation + DEAE-cellulose chromatography, gradient elution.
State:
Lyophilized purified Ascites
Purified
Reconstitution:
Reconstitute with 1 ml sterile water and add preservative if preferred.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:

The antibody reacts with a protease sensitive surface antigen that is present on human endothelial cells of various origins: microvasculature, aorta and arterial and venous umbilical vessels.
Crossreactions with blood cells or plasma proteins were not found.
The antibody-antigen complex is rapidly internalized by metabolically active endothelial cells (at 37°C within 15-30 min.). Also liposomes coated with the antibody are rapidly internalized by endothelial cells. The internalization does not affect the growth and morphological appearance of endothelial cells in culture. The internalization of the antibody is not dependent on the Fc part of the molecule. Specific technical details will dependent on the methods to be used.

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