BM4057B Endothelial Cells antibody

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0.2 mg / €480.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rat anti Human Endothelial Cells 1F10


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  • BM4057B

Product Description for Endothelial Cells

Rat anti Human Endothelial Cells 1F10.
Presentation: Biotin
Product is tested for Frozen Sections.

Properties for Endothelial Cells

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Endothelial Cell marker
Presentation Biotin
Reactivity Hu
Applications C
Clonality Monoclonal
Clone 1F10
Host Rat
Isotype IgG2a
Shipping to USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Cultured HUVECs
Application Immunohistochemistry on Frozen sections: 2 µg/ml (1:200).
Positive control: Human placenta.
Does not react on routinely processed paraffin sections.
Background The antigen is found in variable numbers on freshly isolated umbilical vein endotheli al cells (HUVECs). Antigen expression on cultured HUVECs is dependent on the culture conditions. Best results were obtained (40 - 70% pos.) by daily renewal of FCS containing medium supplemented with endothelial cell conditioned supernatant whereas commercia lly available endothelial cell media apparently have no influence on the antigen expression.
General Readings
  1. Goerdt S, Steckel F, Schulze-Osthoff K, Hagemeier HH, Macher E, Sorg C. Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. Exp Cell Biol. 1989;57(4):185-92. PubMed PMID: 2599260.
Storage Store lyophilized at 2-8°C.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Phosphate buffered saline pH 7.2
0.01% thimerosal
10 mg/ml bovine serum albumin
Lyophilized affinity purified Ig fraction
Reconstitute by adding 0.5 ml distilled water.
Species Reactivity
Species reactivity (tested):
Human. Negative on Mouse.
This antibody recognizes an antigen on human continuous blood endothelial cells which are fully differentiated. The antibody staining is restricted to endothelial cells and does not sta in any other cell in tissues or in peripheral blood. On sinusoidal endothelial cells of the liver or lymphatic organs the antigen is inconsistently expressed or absent.

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