discontinued

BM116 EOSINOPHIL MAJOR BASIC PROTEIN antibody

See related secondary antibodies

Search for all "EOSINOPHIL MAJOR BASIC PROTEIN"

Quick Overview

Mouse anti Guinea Pig, Human, Rat EOSINOPHIL MAJOR BASIC PROTEIN BMK-13

BM116

Product Description for EOSINOPHIL MAJOR BASIC PROTEIN

Mouse anti Guinea Pig, Human, Rat EOSINOPHIL MAJOR BASIC PROTEIN BMK-13.
Product is tested for Frozen Sections, Enzyme Immunoassay.

Properties for EOSINOPHIL MAJOR BASIC PROTEIN

Product Category Primary Antibodies
Quantity 1 ml
Reactivity GP, Hu, Rt
Applications C, E
Clonality Monoclonal
Clone BMK-13
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Add. information * Staining requires antigen retrieval with pepsin. Do not use heat induced retrieval methods.
Application Immunohistochemistry on frozen and paraffin* sections: 1/15 - 1/30.
ELISA.
Caution (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Concentration 10 µg/ml
General Readings
  1. Moqbel R, Barkans J, Bradley BL, Durham SR, Kay AB. Application of monoclonal antibodies against major basic protein (BMK-13) and eosinophil cationic protein (EG1 and EG2) for quantifying eosinophils in bronchial biopsies from atopic asthma. Clin Exp Allergy. 1992 Feb;22(2):265-73. PubMed PMID: 1373987.
  2. Haczku A, Moqbel R, Jacobson M, Kay AB, Barnes PJ, Chung KF. T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure. Immunology. 1995 Aug;85(4):591-7. PubMed PMID: 7558154. (Free PMC Article available)
  3. Hashimoto Y, Nagaoka I, Yamashita T. Purification of the antibacterial fragments of guinea-pig major basic protein. Biochim Biophys Acta. 1993 Dec 8;1203(2):236-42. PubMed PMID: 8268206.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Tissue Culture Supernatant containing 0.09% Sodium azide
Specificity
Specificity:
This antibody recognises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). The antibody stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a "Pan eosinophil" marker. The clone cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.
Species:
Human, Rat, Guinea Pig.

Accessory Products

  • LinkedIn