discontinued

BM116 EOSINOPHIL MAJOR BASIC PROTEIN antibody

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1 ml / €370.00

Quick Overview

Mouse anti Guinea Pig, Human, Rat EOSINOPHIL MAJOR BASIC PROTEIN BMK-13

BM116

Product Description for EOSINOPHIL MAJOR BASIC PROTEIN

Mouse anti Guinea Pig, Human, Rat EOSINOPHIL MAJOR BASIC PROTEIN BMK-13.
Product is tested for Frozen Sections, Enzyme Immunoassay.

Properties for EOSINOPHIL MAJOR BASIC PROTEIN

Product Category Primary Antibodies
Quantity 1 ml
Reactivity GP, Hu, Rt
Applications C, E
Clonality Monoclonal
Clone BMK-13
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Add. information * Staining requires antigen retrieval with pepsin. Do not use heat induced retrieval methods.
Application Immunohistochemistry on frozen and paraffin* sections: 1/15 - 1/30.
ELISA.
Caution (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Concentration 10 µg/ml
General Readings
  1. Moqbel R, Barkans J, Bradley BL, Durham SR, Kay AB. Application of monoclonal antibodies against major basic protein (BMK-13) and eosinophil cationic protein (EG1 and EG2) for quantifying eosinophils in bronchial biopsies from atopic asthma. Clin Exp Allergy. 1992 Feb;22(2):265-73. PubMed PMID: 1373987.
  2. Haczku A, Moqbel R, Jacobson M, Kay AB, Barnes PJ, Chung KF. T-cells subsets and activation in bronchial mucosa of sensitized Brown-Norway rats after single allergen exposure. Immunology. 1995 Aug;85(4):591-7. PubMed PMID: 7558154. (Free PMC Article available)
  3. Hashimoto Y, Nagaoka I, Yamashita T. Purification of the antibacterial fragments of guinea-pig major basic protein. Biochim Biophys Acta. 1993 Dec 8;1203(2):236-42. PubMed PMID: 8268206.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Tissue Culture Supernatant containing 0.09% Sodium azide
Specificity
Specificity:
This antibody recognises the Eosinophil Major Basic Protein (EMBP), a 117 amino acid protein, corresponding to residues 106-222 of Bone marrow proteoglycan (precursor). The antibody stains both resting and activated eosinophils of bronchial and skin sections of allergic and normal sites and may be considered a "Pan eosinophil" marker. The clone cross reacts weakly with basophils which also contain low levels of EMBP. No cross reactivity with other human cells or proteins has been noted.
Species:
Human, Rat, Guinea Pig.

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