AM50268PU-T Eosinophil peroxidase antibody

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20 µg / €180.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Eosinophil peroxidase EPO104

AM50268PU-T

Product Description for Eosinophil peroxidase

Mouse anti Human Eosinophil peroxidase EPO104.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Eosinophil peroxidase

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms EPER, EPO, EPP, EPX
Presentation Purified
Reactivity Hu
Applications C, ICC/IF
Clonality Monoclonal
Clone EPO104
Host Mouse
Isotype IgG1
Molecular weight 70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11678
Immunogen:
Human Eosinophils from a patient with hypereosinophilic syndrome.
GeneID:
8288
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: For coating, order Ab without BSA.
Flow Cytometry: 0.5-1 µg/million cells. 
Immunofluorescence: 0.5-1 µg/ml.
Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 minutes at RT.
Positive Control: MCF-7 cells, Tonsil.
Background Peripheral blood granulocytes are classified into neutrophil s, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both norm al and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil.
Concentration 0.2 mg/ml
General Readings
  1. Abbas, A., A.H. Lichtman and J.S. Pober. 1994. Cellular and Molecular Biology, W.B. Saunders Co., Philadelphia, London, Toronto, Tokyo), pp 14-30.
  2. Skubitz KM, Christiansen NP, Mendiola JR. Preparation and characterization of monoclonal antibodies to human neutrophil cathepsin G, lactoferrin, eosinophil peroxidase, and eosinophil major basic protein. J Leukoc Biol. 1989 Aug;46(2):109-18. PubMed PMID: 2746138.
  3. Skubitz KM, Wehner NG, Gray BH. Preparation and characterization of a monoclonal antibody that inhibits human neutrophil elastase activity. J Leukoc Biol. 1988 Sep;44(3):158-65. PubMed PMID: 3411232.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This Monoclonal Antibody stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). 
Recognizes Human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. 
Cellular Localization: Cytoplasmic, granular.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Eosinophil peroxidase (3 products)

Catalog No. Species Pres. Purity   Source  

Eosinophil peroxidase

Eosinophil peroxidase Human Purified >95% pure by SDS-PAGE. Eosinophils
0.2 mg / €1,070.00
  OriGene Technologies GmbH

Eosinophil peroxidase

Eosinophil peroxidase Human Purified
  Abnova Taiwan Corp.

Eosinophil peroxidase

Eosinophil peroxidase Human Purified
  Abnova Taiwan Corp.

Positive controls for Eosinophil peroxidase (1 products)

Catalog No. Species Pres. Purity   Source  

EPX Lysate

Western Blot: EPX Lysate [NBL1-10306] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for EPX
  Novus Biologicals Inc.
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