AP55721PU-N EPHA2/3/4 pTyr588/596 antibody

See related secondary antibodies

Search for all "EPHA2/3/4"

0.1 ml / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse EPHA2/3/4

AP55721PU-N

More Views

  • AP55721PU-N

Product Description for EPHA2/3/4

Rabbit anti Human, Mouse EPHA2/3/4.
Properties: pTyr588/596
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for EPHA2/3/4

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms ECK, EK4, Eph receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, TYRO4, Tyrosine-protein kinase receptor ECK/ ETK1
Presentation Aff - Purified
Reactivity Hu, Ms
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 130 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P29317
Immunogen:
Peptide sequence around phosphorylation site of tyrosine 588/596 (K-T-Y(p)-V-R)/(R-T-Y(p)-V-D) derived from Human EPHA2/3/4 (KLH-conjugated)
GeneID:
1969
Property pTyr588/596
Application Western blot: 1:500~1:1000.
Immunofluorescence: 1:100~1:200.
Background Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.
Concentration 1.0 mg/ml
General Readings
  1. Lindberg RA, Hunter T. cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases. Mol Cell Biol. 1990 Dec;10(12):6316-24. PubMed PMID: 2174105. (Free PMC Article available)
  2. Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, et al. Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11. PubMed PMID: 15951569.
  3. Zhuang G, Hunter S, Hwang Y, Chen J. Regulation of EphA2 receptor endocytosis by SHIP2 lipid phosphatase via phosphatidylinositol 3-Kinase-dependent Rac1 activation. J Biol Chem. 2007 Jan 26;282(4):2683-94. Epub 2006 Nov 29. PubMed PMID: 17135240.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography using epitope-specific peptide
Buffer System:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse
Specificity
Specificity:
The antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596

Accessory Products

Proteins and/or Positive Controls

Proteins for EPHA2/3/4 (12 products)

Catalog No. Species Pres. Purity   Source  

EPHA2 / ECK

EPHA2 / ECK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

EPHA2 / ECK (C-term DDK/His tag)

EPHA2 / ECK Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EPHA2 / ECK (C-term DDK tag)

EPHA2 / ECK Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

EPHA2 / ECK

EPHA2 / ECK Human Purified
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human Purified
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human
  Abnova Taiwan Corp.

EPHA2 / ECK

EPHA2 / ECK Human
  Abnova Taiwan Corp.

Positive controls for EPHA2/3/4 (2 products)

Catalog No. Species Pres. Purity   Source  

EPHA2 293T Cell Transient Overexpression Lysate(Denatured)

EPHA2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

EPHA2 293T Cell Transient Overexpression Lysate(Denatured)

EPHA2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
  • LinkedIn