AP05620PU-N Ephrin-B pTyr298 antibody

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Rabbit anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus, Zebrafish Ephrin-B


Product Description for Ephrin-B

Rabbit anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus, Zebrafish Ephrin-B.
Properties: pTyr298
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Ephrin-B

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Purified
Reactivity Bov, Can, Chk, Hu, Mky, Ms, Rt, Xen, Ze
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic phosphotide corresponding to an amino acid sequence within Xenopus Ephrin B, which includes phosphorylated Tyr298.
Xenopus Tyr298 is the homolog of Human, Mouse and Rat Tyr317 and also Chicken Tyr305.
Property pTyr298
Application Western Blot: 1/1000. Detects a band of approximately 46kDa in rat testes cell lysates.
Background Ephrin B is a 46 kDa member of the ephrin family, expressed on cell membranes and acts as a ligand for ephrin B family receptor tyrosine kinases. Ephrin B plays an important role in, directing thalamocortical axon growth specifically through intra-areal topographic mapping as well as controlling more regional, inter-areal growth. Ephrin B is also crucial to vasculogenesis, angiogenesis, and blood vessel maturation.
Binding ephrin B family receptors on target cells causes bidirectional signalling, not only initiating signalling in the target cell but also causing a reverse signal in the ephrin B expressing cell.
General Readings
  1. Dufour A, Seibt J, Passante L, Depaepe V, Ciossek T, Frisén J, et al. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes. Neuron. 2003 Jul 31;39(3):453-65. PubMed PMID: 12895420.
  2. Oike Y, Ito Y, Hamada K, Zhang XQ, Miyata K, Arai F, et al. Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells. Blood. 2002 Aug 15;100(4):1326-33. PubMed PMID: 12149214.
  3. Palmer A, Zimmer M, Erdmann KS, Eulenburg V, Porthin A, Heumann R, et al. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Mol Cell. 2002 Apr;9(4):725-37. PubMed PMID: 11983165.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography.
Buffer System:
10mM Hepes pH 7.5 containing 0.09% Sodium Azide as preservative and 50% Glycerol, 0.01% BSA as stabilizers.
Liquid purified IgG fraction.
Species Reactivity
Species reactivity (expected):
Zebrafish, Mouse, Canine (Dog), Primate, Human, Bovine and Chicken.
Species reactivity (tested):
Xenopus and Rat.
This antibody recognises Ephrin B phosphorylated at Tyrosine residue 298.

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