AM06412SU-N ERK1 / ERK2 antibody

See related secondary antibodies

Search for all "ERK1 / ERK2"

0.1 ml / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Mouse ERK1 / ERK2 3F8


More Views

  • AM06412SU-N

Product Description for ERK1 / ERK2

Mouse anti Human, Mouse ERK1 / ERK2 3F8.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAP
Presentation Ascites
Reactivity Hu, Ms
Applications E, P, WB
Clonality Monoclonal
Clone 3F8
Host Mouse
Isotype IgG2b
Molecular weight 42 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified recombinant fragment of human MAPK expressed in E. Coli.
Application ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Flow Cytometry: 1/200 - 1/400.
Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Background Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.
General Readings
  1. FEBS Lett. 1992 Jun 15;304(2-3):170-8.
  2. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5361-5.
  3. J Biol Chem. 2009 Nov 27;284(48):33456-65.
  4. BMC Biol. 2009 Oct 27;7:70.

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Ascitic fluid containing 0.03% Sodium Azide.
Species Reactivity
Species reactivity (tested):
Human, Mouse.
Recognizes p44/42 MAPK (Erk1/2).

Accessory Products

  • LinkedIn