AP00033PU-N ERK1 / ERK2 antibody

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Rabbit anti Chicken, Human, Mouse, Rat, Sheep ERK1 / ERK2

Product Description for ERK1 / ERK2

Rabbit anti Chicken, Human, Mouse, Rat, Sheep ERK1 / ERK2.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Chk, Hu, Ms, Rt, Sh
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified cytochrome p450
Application Western blot: 0.5-4 µg/ml.
Immunoprecipitation: 20-40 µg/ml.
Immunohistochemistry: 20-40 µg/ml.
Background The extracellular signal-regulated kinase 1 and 2 (Erk1 and Erk2) are closely related mitogen activated protein (MAP) kinases that are activated through the extracellular stimulation by many growth factors, mitogens and differentiation promoting agents via a protein kinase cascade. Erk1/2 are activated approximately 1000 fold by phosphorylation of neibouring threonine and tyrosine residues by Mek1 and Mek2. Both sites must be phosphorylated for maximum activity. Erk1/2 kinases are ubiquitously distributed in the nervous system, as well as a broad range of cells and tissues. These two kinases represent proximal kinases in the classical MAP kinase cascade pathway which links growth and differentiation signals at the cell surface (through tyrosine kinase) with transcription in the nucleus.
Concentration 0.5 mg/ml
Storage Store the antibody at -20°C
For long-term storage, aliquot and freeze at -70°C.
Avoid repeated freeze/defrost cycles.
Shelf life: one year from despatch.
Affinity Chromatography.
Buffer System:
PBS, pH 7.2 containing 50% Glycerol, 1% BSA and 0.02% Thimerosal.
Liquid purified Ig fraction.
Aff - Purified
The antibody recognizes 42 kDa and 44 kDa of endogeneous Erk1/Erk2 (p44/p42 MAP kinase).
Human, Mouse, Rat, Sheep, Chicken and Hamster.

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