AP01886PU-N ERK1 / ERK2 pTyr204 antibody

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0.1 mg / €350.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat ERK1 / ERK2

AP01886PU-N

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Product Description for ERK1 / ERK2

Rabbit anti Human, Mouse, Rat ERK1 / ERK2.
Properties: pTyr204
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 44,42 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Tyrosine 204.
Property pTyr204
Application Western Blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Immunofluorescence: 1/50-1/200.
Background The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen.
Buffer System:
Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of ERK1/2 protein when phosphorylated at Tyr204.

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