AP02499PU-N ERK1 / ERK2 pThr187/pTyr204 antibody

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0.1 ml / €320.00
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Rabbit anti Human, Mouse, Rat ERK1 / ERK2

AP02499PU-N

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Product Description for ERK1 / ERK2

Rabbit anti Human, Mouse, Rat ERK1 / ERK2.
Properties: pThr187/pTyr204
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).
Property pThr187/pTyr204
Application Western Blot: 1/500~1/1000.
Immunohistochemistry: 1/50~1/100.
Immunofluorescence: 1/100~1/200.
Background Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
Concentration 1.0 mg/ml
Protocols 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
2. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
3. Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
4. Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.
Species:
Human, Mouse and Rat.
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