AP02499PU-N ERK1 / ERK2 pThr187/pTyr204 antibody

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0.1 ml / €320.00
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Rabbit anti Human, Mouse, Rat ERK1 / ERK2


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  • AP02499PU-N
  • AP02499PU-N

Product Description for ERK1 / ERK2

Rabbit anti Human, Mouse, Rat ERK1 / ERK2.
Properties: pThr187/pTyr204
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).
Property pThr187/pTyr204
Application Western Blot: 1/500~1/1000.
Immunohistochemistry: 1/50~1/100.
Immunofluorescence: 1/100~1/200.
Background Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
Concentration 1.0 mg/ml
Protocols 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
2. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
3. Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
4. Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
Liquid purified Ig fraction.
Aff - Purified
This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.
Human, Mouse and Rat.
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