AP10012PU-N ERK1 / ERK2 antibody

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Rabbit anti Human, Mouse, Rat ERK1 / ERK2 B20-U

Product Description for ERK1 / ERK2

Rabbit anti Human, Mouse, Rat ERK1 / ERK2 B20-U.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone B20-U
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Manufacturer certificates ISO 13485:2003, ISO 9001:2008
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Peptide derived from C-terminal sequence of Human Erk-2
Application Immunohistochemistry (Frozen Sections, Fixed, Paraffin-Embedded Sections).
ELISA (1/20,000-1/100,000).
Western blot (1/5,000)
Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Triton X-100
Blocking buffer: 1xTBS; 0.2% Triton X-100; 8% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature.
Background ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signalling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK 1 and ERK 2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK 1 + 2 enzymes
Concentration 4.0 mg/ml
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Immunoaffinity Chromatography.
Buffer System:
20 mM Tris-HCl, pH 8.0 containing 0.05% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer.
Note: This antibody is aslo available without Stabilizer.
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse and Rat.
This antibody recognises Erk1,2.
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