AP23276PU-N ERK1 / ERK2 (N-term) antibody

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0.1 mg / €340.00
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Rabbit anti Human, Mouse, Rat ERK1 / ERK2

AP23276PU-N

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  • AP23276PU-N

Product Description for ERK1 / ERK2

Rabbit anti Human, Mouse, Rat ERK1 / ERK2.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications IP, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide mapping at the N-terminal of the human MAPK1+3
Remarks:
Sequence is identical to the related rat sequence.
Property N-term
Application Western blot: At 0.5-1μg/ml with the appropriate system to detect MAPK1/3 in cells and tissues.
Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect MAPK1/3 in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
Background MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2.
Storage Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunogen affinity purified
Buffer System:
5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
State:
Lyophilized Ig fraction
Aff - Purified
Reconstitution:
0.2ml of distilled water will yield a concentration of 500μg/ml.
Species Reactivity
Species reactivity (tested):
Human, mouse, rat
Specificity
Specificity:
This antibody detects ERK1 / ERK2 at N-term. No cross reactivity with other proteins.

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