NB110-96887 ERK1 / ERK2 antibody

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Rabbit anti Bovine, Chicken, Drosophila, Human, Mouse, Rat, Sheep, Xenopus ERK1 / ERK2

Product Description for ERK1 / ERK2

Rabbit anti Bovine, Chicken, Drosophila, Human, Mouse, Rat, Sheep, Xenopus ERK1 / ERK2.
Presentation: Serum
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
Presentation Serum
Reactivity Bov, Chk, Dros, Hu, Ms, Rt, Sh, Xen
Applications F, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Add. information Research Areas: Lipid and Metabolism, Cancer
Application flow cytometry / FACS analysis, immunohistochemistry, Western Blot, Flow cytometry / FACS analysis, Western Blot and Immunohistochemistry , flow cytometry / FACS analysis 10 ug/mL, immunohistochemistry 1:50, Western Blot 1:5000 (ECL)
Background The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Whole antisera
Buffer System:
No Preservative
Whole antisera
Detects approx. 44Kda and approx. 42kDa bands corresponding to the molecular weights of Erk1 and Erk2.
Bovine, Chicken, Drosophila, Human, Mouse, Rat, Sheep, Xenopus

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