NBP1-28628 ERK1 / ERK2 antibody

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Mouse anti Human ERK1 / ERK2 SB46b

Product Description for ERK1 / ERK2

Mouse anti Human ERK1 / ERK2 SB46b.
Presentation: HRP
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 1 ml
Synonyms ERK-1, ERK-2, ERK1, ERK2, ERT1, ERT2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1, MAPK3, Mitogen-activated protein kinase, PRKM1, PRKM2, PRKM3
Presentation HRP
Reactivity Hu
Applications IP, WB
Clonality Monoclonal
Clone SB46b
Host Mouse
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Application immunoprecipitation, Western Blot, Immunoprecipitation and Western Blot, immunoprecipitation , Western Blot
Background Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases (2-5), Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding 5. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2 (5,6). Both sites must be phosphorylated for maximum activity (2-5). These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus (2-4). The SB46b monoclonal antibody precipitates approx. 42 kDa and approx. 44 kDa bands, corresponding to Erk1 and Erk2, respectively.
Storage Store at 4C. Do not freeze.
IgG purified
Buffer System:
No Preservative
IgG purified
Reacts with human GFAP (approx. 50 kDa) (1)

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