discontinued

NSB654 ERK1 / ERK2 antibody

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0.1 ml / €390.00

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Rabbit anti Bovine, Human, Mouse, Rat ERK1 / ERK2

Product Description for ERK1 / ERK2

Rabbit anti Bovine, Human, Mouse, Rat ERK1 / ERK2.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for ERK1 / ERK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2, P42/P44-MAPK
Presentation Aff - Purified
Reactivity Bov, Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide, corresponding to C terminal amino acids 317-339 of Human ERK1 + ERK2.
Add. information Research Areas: Cancer, Lipid and Metabolism
Application Western Blot, WB: Predicted molecular weight: ~42-44 kDa. Optimal dilutions/concentrations should be determined by the end user., Western Blot 1/1000
Background ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.
General Readings General / background references:Tanimura S et al. Prolonged nuclear retention of activated extracellular signal-regulated kinase 1/2 is required for hepatocyte growth factor-induced cell motility. J Biol Chem 277:28256-64 (2002). PubMed PMID: 12032150
Janulis M et al. A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity. Mol Cell Biol 21:2235-47 (2001). PubMed PMID: 11238956
Widmann C et al. Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev 79:143-80 (1999). PubMed PMID: 9922370
Khokhlatchev A et al. Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases. J Biol Chem 272:11057-62 (1997). PubMed PMID: 9110999
Boulton TG & Cobb MH Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies. Cell Regul 2:357-71 (1991). PubMed PMID: 1654126
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
ERK1 + ERK2
Species:
Bovine, Human, Mouse, Rat

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