AP33080AF-N Erythrocytes antibody

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10 mg / €280.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Sheep Erythrocytes

AP33080AF-N

Product Description for Erythrocytes

Rabbit anti Sheep Erythrocytes.
Presentation: Azide Free
Product is tested for Immunocytochemistry/Immunofluorescence, Dot blot, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Erythrocytes

Product Category Primary Antibodies
Quantity 10 mg
Presentation Azide Free
Reactivity Sh
Applications Dot, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Not required.
Application ELISA. 
Dot blot.
Immunoblotting.
Indirect Immunofluorescence. 

Can be used to sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
Antibody titre: Hemolytic titre at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a dilution of 1/60) and 108 RBC/ml not less than 1/3,000 when tested with Sheep Erythrocytes.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
Preservatives:
None, as it may interfere with the antibody activity.
Stabilizers:
None
State:
Lyophilized purified hyperimmune IgG fraction
Azide Free
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
Sheep Erythrocytes.
Cross-reactivity: Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.

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