discontinued

AM32741PU-N Erythroid Cells CSA (Nucleated) antibody

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0.1 mg / €380.00

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Mouse anti Human Erythroid Cells CSA HAE9

AM32741PU-N

Product Description for Erythroid Cells CSA

Mouse anti Human Erythroid Cells CSA HAE9.
Properties: (Nucleated)
Presentation: Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for Erythroid Cells CSA

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications F, IP
Clonality Monoclonal
Clone HAE9
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human fetal liver cells
Property Nucleated
Isotype control SM13P
Application Immunoprecipitation: 5 μg/500 μl of cell lysate in 0.5% triton X-100 diluted in PBS with proteinase inhibitors.
Flow Cytometry: 10 μg/ml concentration (1/100); per 106 cells, 100 μl sample volume.
Concentration 1.0 mg/ml
General Readings
  1. Mechetner EB, Sedmak DD, Barth RF. Heterogeneity of peripheral blood reticulocytes: a flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange. Am J Hematol. 1991 Sep;38(1):61-3. PubMed PMID: 1716850.
  2. Tupitsyn NN, Mechetner EB, Baryschnikov AJu, Drozdova TS, Frenkel MA, Ievleva ES, et al. Two different anti-erythroid monoclonal antibodies in immunodiagnosis of human leukemias: a comparative study. Int J Cancer. 1989 Oct 15;44(4):589-92. PubMed PMID: 2676869.
  3. Mechetner EB, Tonevitsky AG, Ievleva ES, Rozinova EN, Popova ON. Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9. Exp Hematol. 1987 May;15(4):355-9. PubMed PMID: 3569438.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Gel Filtration from tissue culture supernatants
Buffer System:
20mM PB pH 7.6, 250mM NaCl
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
Cell Surface Antigen of Human Nucleated Erythroid Cells. AM32741PU-N recognizes a 70 kDa surface protein in about 100% nucleated erythroid cells (erythroblasts, normoblasts, proerythroblasts), 36% of late erythroid committed cells and 70-80% of fetal liver cells. Normal stem cells, early erythroid committed cells and hematopoetic non-erythroid cells do not react with this antibody AM32741PU-N.
The 70 kDa protein may be associated with an isoform of transferrin specific for red cells.
Applicable for immunostaining and enumeration of nucleated erythroid cells in bone marrow, fetal liver or cord blood and immunophenotyping human leukemias (Mechetner, EB et al. 1987). Identification of a Human erythroid cell surface antigen by monoclonal antibody HAE9 (Exp Hematol. 15(4):355-9).
Negative Species: Mouse and Rat.

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