AP60003PU-N ESM1 antibody

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0.1 mg / €270.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Mouse ESM1

Product Description for ESM1

Rabbit anti Mouse ESM1.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for ESM1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Endocan, Endothelial cell-specific molecule 1
Presentation Purified
Reactivity Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9QYY7
Immunogen:
Highly pure (>95%) recombinant human Endocan/ESM1 (Trp19-Arg184) derived from E. coli
GeneID:
71690
Application Western blot: 1-5 μg/ml.
Background Endocan, also known as endothelial cell-specific molecule1 (ESM1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung. Endocan expression has also been detected in different epithelia and in adipocytes. The expression of endocan is up-regulated by TNFα, IL1β or lipopolysaccharide and down-regulated by IFNγ. The mouse Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues. The DS chain is covalently attached to serine 137. Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen1, LFA1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM1 and reducing LFA1mediated leukocyte activation. Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing Endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo. Circulating Endocan can be detected in the serum from healthy subjects.
General Readings
  1. Lassalle P, Molet S, Janin A, Heyden JV, Tavernier J, Fiers W, et al. ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J Biol Chem. 1996 Aug 23;271(34):20458-64. PubMed PMID: 8702785.
  2. Bechard D, Meignin V, Scherpereel A, Oudin S, Kervoaze G, Bertheau P, et al. Characterization of the secreted form of endothelial-cell-specific molecule 1 by specific monoclonal antibodies. J Vasc Res. 2000 Sep-Oct;37(5):417-25. PubMed PMID: 11025405.
  3. Wellner M, Herse F, Janke J, Gorzelniak K, Engeli S, Bechart D, et al. Endothelial cell specific molecule-1--a newly identified protein in adipocytes. Horm Metab Res. 2003 Apr;35(4):217-21. PubMed PMID: 12778364.
  4. Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, et al. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001 Dec 21;276(51):48341-9. Epub 2001 Oct 5. PubMed PMID: 11590178.
  5. Béchard D, Scherpereel A, Hammad H, Gentina T, Tsicopoulos A, Aumercier M, et al. Human endothelial-cell specific molecule-1 binds directly to the integrin CD11a/CD18 (LFA-1) and blocks binding to intercellular adhesion molecule-1. J Immunol. 2001 Sep 15;167(6):3099-106. PubMed PMID: 11544294.
  6. Scherpereel A, Gentina T, Grigoriu B, Sénéchal S, Janin A, Tsicopoulos A, et al. Overexpression of endocan induces tumor formation. Cancer Res. 2003 Sep 15;63(18):6084-9. PubMed PMID: 14522939.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein A Chromatography
Buffer System:
5 mM PBS, pH 7.2
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody detects Mouse Endocan / ESM1.
Other species not tested.

Accessory Products

Proteins and/or Positive Controls

Proteins for ESM1 (1 products)

Catalog No. Species Pres. Purity   Source  

ESM1 (His-tag)

SDS-PAGE analysis of recombinant mouse Endocan / ESM1 derived from insect cells Cat.-No. AR60003PU-N. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain. Mouse Purified > 95 % by SDS-PAGE and Silver staining Insect cells
50 µg / €350.00
  OriGene Technologies GmbH
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