discontinued

AP09861PU-N Estrogen receptor beta (Isoform 1) antibody

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Rabbit anti Mouse, Rat Estrogen receptor beta

Product Description for Estrogen receptor beta

Rabbit anti Mouse, Rat Estrogen receptor beta.
Properties: (Isoform 1)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Estrogen receptor beta

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms ER-beta, ESR2, ESTRB, NR3A2, Nuclear receptor subfamily 3 group A member 2
Presentation Aff - Purified
Reactivity Ms, Rt
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O08537
Immunogen:
Synthetic peptide corresponding to unique epitope on Estrb-1.
Remarks:
The Estrb-1 peptide was post-synthetically covalently modified to achieve desired antigenicity.
Property Isoform 1
Add. information Antigenic blocking peptide (AP09861CP-N) for Estrb-1 is also available separately.
Application Suitable for use in ELISA (< 1/10,000) and Western Blot (> 1/500).
Background Estrogen, a steroid hormone, is a key regulator of growth and differentiation in a broad range of target tissues and, it is also implicated in breast and endometrial cancer and osteoporosis (1). Like all steroid hormones, estrogen readily diffuses across cell membrane and binds to and activates estrogen receptors (α and β) which then up-regulate the expression of many genes. These receptors belong to the superfamily of nuclear receptors, more specifically to the family of steroid receptors that act as ligand-regulated transcription factors (2). Estrogen binding receptor protein was first discovered in the early sixties, followed by cloning of this receptor in the mid-eighties. In 996, this receptor was renamed estrogen receptor alpha (ER-alpha) due to cloning of an additional estrogen receptor from rat prostrate. This novel receptor was designated estrogen receptor beta (ER-beta) (3). ER-beta is located on mouse chromosome 12 D1-D3. Recent reports have shown that ER-beta is important for the prevention of age-related hearing loss, proper cAMP signaling pathway, polyovular follicles in mouse ovaries and also important in regulating tyrosine hydroxylase expression in the moue locus coeruleus (4).
Any disruption in estrogen receptor beta signaling has been shown to cause negative effects on ovulation and fertility (4). ER-beta deficiency in mice was found to be associated with increase in small intestine tumorigenesis. ER-beta deficiency also leads to increase epithelial proliferation, decreased apoptosis, and accumulation of incompletely differentiated cells in mouse ventral prostrates (5). In male mice, ER-beta deletion affected the structure of their arteries. ER-beta is expressed in kidney, spleen, testis and skeletal muscles. It is approximately a 72kDa protein (567 amino acids).
Concentration 0.64-0.72 mg/ml (lot specific)
General Readings
  1. Petterson K, Gustafsson JA. Role of estrogen receptor beta in estrogen action. Annu Rev Physiol. 2001; 63:165-192.
  2. Beato M. Gene regulation by steroid hormones. Cell. 1989; 56:335-344.
  3. Kuiper GG, et al. Cloning of a novel receptor expressed in rat prostrate and ovary. Proc Natl Acad Sci USA. 1996; 93:5925-5930.
  4. Pendergast JS, Tuesta LM, Bethea JR. Oestrogen receptor beta contributes to the transient sex difference in tyrosine hydroxylase expression in the mouse locus coeruleus. J Neuroendocrinol. 2008 Oct;20(10):1155-64. doi: 10.1111/j.1365-2826.2008.01776.x. Epub 2008 Jul 31. PubMed PMID: 18680559.
  5. Deroo BJ, Rodriguez KF, Couse JF, Hamilton KJ, Collins JB, Grissom SF, et al. Estrogen receptor beta is required for optimal cAMP production in mouse granulosa cells. Mol Endocrinol. 2009 Jul;23(7):955-65. doi: 10.1210/me.2008-0213. Epub 2009 Mar 26. PubMed PMID: 19324971. (Free PMC Article available, 6 images available)
  6. Imamov O, Morani A, Shim GJ, Omoto Y, Thulin-Andersson C, Warner M, et al. Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9375-80. Epub 2004 Jun 8. PubMed PMID: 15187231. (Free PMC Article available, 7 images available)
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on immobilized antigen
Buffer System:
Stabilization buffer
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody detects a single band of approximately 72kDa in Western blot positive control samples for Estrb-1 and several other tissues.
Species:
Mouse and Rat.

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