discontinued

SM1682X Estrogen receptor beta (Isoform 1) (C-term), 10 x conc. antibody

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Mouse anti Human Estrogen receptor beta PPG5/10

SM1682X

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Product Description for Estrogen receptor beta

Mouse anti Human Estrogen receptor beta PPG5/10.
Properties: (Isoform 1) (C-term), 10 x conc.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Paraffin Sections.

Properties for Estrogen receptor beta

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms ER-beta, ESR2, ESTRB, NR3A2, Nuclear receptor subfamily 3 group A member 2
Presentation Supernatant
Reactivity Hu
Applications C, ICC/IF, P
Clonality Monoclonal
Clone PPG5/10
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q92731
Immunogen:
Synthetic peptide derived from the C-terminus of the Human oestrogen receptor beta 1 isoform.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
AA Sequence:
CEDSKSKEGSQNPQSQ
GeneID:
2100
Property 10 x conc., C-term, Isoform 1
Application Immunofluorescence. 
Immunohistochemistry on Frozen Sections.

Immunohistochemistry on Paraffin Sections: 1/5-1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Recommended Positive Control: Ovary. 
Clone PPG5/10 has also been reported to be suitable for Western blotting.
Background Estrogen Receptor beta is a recent discovery, initially cloned and characterized from testis. Estrogen Receptor beta is highly homologous to Estrogen Receptor alpha; the ligand and DNA binding domains are highly conserved, while the amino terminus (transactivation domain), has diverged significantly. Estrogen Receptor beta binds estrogen with high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (EREs).
General Readings
  1. Moore JT, McKee DD, Slentz-Kesler K, Moore LB, Jones SA, Horne EL, et al. Cloning and characterization of human estrogen receptor beta isoforms. Biochem Biophys Res Commun. 1998 Jun 9;247(1):75-8. PubMed PMID: 9636657.
  2. Saunders PT, Millar MR, Williams K, Macpherson S, Harkiss D, Anderson RA, et al. Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans. Biol Reprod. 2000 Oct;63(4):1098-105. PubMed PMID: 10993832.
  3. Torlakovic E, Lilleby W, Torlakovic G, Fosså SD, Chibbar R. Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score. Hum Pathol. 2002 Jun;33(6):646-51. PubMed PMID: 12152165.
  4. Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, et al. Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies. Br J Cancer. 2002 Jan 21;86(2):250-6. PubMed PMID: 11870515. (Free PMC Article available, 5 images available)
  5. Wong NA, Malcomson RD, Jodrell DI, Groome NP, Harrison DJ, Saunders PT. ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates. J Pathol. 2005 Sep;207(1):53-60. PubMed PMID: 15954165.
  6. Fixemer T, Remberger K, Bonkhoff H. Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma. Prostate. 2003 Feb 1;54(2):79-87. PubMed PMID: 12497580.
  7. Wood CE, Usborne AL, Starost MF, Tarara RP, Hill LR, Wilkinson LM, et al. Hyperplastic and neoplastic lesions of the mammary gland in macaques. Vet Pathol. 2006 Jul;43(4):471-83. PubMed PMID: 16846989.
  8. Juengel JL, Heath DA, Quirke LD, McNatty KP. Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep. Reproduction. 2006 Jan;131(1):81-92. PubMed PMID: 16388012.
  9. Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, et al. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330. doi: 10.1186/1471-2407-9-330. PubMed PMID: 19758455. (Free PMC Article available, 5 images available)
  10. Carpino A, Rago V, Pezzi V, Carani C, Andò S. Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor. Eur J Endocrinol. 2007 Aug;157(2):239-44. PubMed PMID: 17656605.
  11. Bianchi C, Sahlin L, Meikle A, Masironi B, Cavilla M, Aba M. Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama). Reprod Domest Anim. 2010 Oct;45(5):872-80. doi: 10.1111/j.1439-0531.2009.01401.x. PubMed PMID: 19548916.
  12. Marotti JD, Collins LC, Hu R, Tamimi RM. Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study. Mod Pathol. 2010 Feb;23(2):197-204. doi: 10.1038/modpathol.2009.158. Epub 2009 Nov 6. PubMed PMID: 19898422. (Free PMC Article available, 3 images available)
  13. Asgari M, Morakabati A. Estrogen receptor beta expression in prostate adenocarcinoma. Diagn Pathol. 2011 Jul 6;6:61. doi: 10.1186/1746-1596-6-61. PubMed PMID: 21733187. (Free PMC Article available, 2 images available)
  14. Fowler PA, Anderson RA, Saunders PT, Kinnell H, Mason JI, Evans DB, et al. Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary. J Clin Endocrinol Metab. 2011 Jun;96(6):1754-62. doi: 10.1210/jc.2010-2618. Epub 2011 Mar 23. PubMed PMID: 21430025.
  15. Mafuvadze B, Benakanakere I, López Pérez FR, Besch-Williford C, Ellersieck MR, Hyder SM. Apigenin prevents development of medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumors in Sprague-Dawley rats. Cancer Prev Res (Phila). 2011 Aug;4(8):1316-24. doi: 10.1158/1940-6207.CAPR-10-0382. Epub 2011 Apr 19. PubMed PMID: 21505181. (Free PMC Article available, 4 images available)
  16. Silvestri A, Fraser HM. Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy. Reproduction. 2007 Aug;134(2):341-53. PubMed PMID: 17660243.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Tissue Culture Supernatant.
This antibody is a 10x concentrate, more suitable for rapid staining procedures than the standard SM1682.
Supernatant
Specificity
Specificity:
This antibody recognises the Estrogen Receptor beta 1 isoform. The antibody has been used for the Immunohistochemical study of the expression of this isoform, showing positive nuclear staining in cells within the ovary, uterus, oviduct, breast, testis, prostate, bladder and male reproductive ducts.
Species:
Human, Cynomolgus Monkey, Rhesus Monkey, New World Primate, Primate, Rat, Sheep, Pig, Marmoset, Llama.
Gene ID 2100
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Estrogen receptor beta (6 products)

Catalog No. Species Pres. Purity   Source  

Estrogen receptor beta (full length, C-term DDK tag)

Estrogen receptor beta Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

Estrogen receptor beta

Estrogen receptor beta Human
  Abnova Taiwan Corp.

Estrogen receptor beta

Estrogen receptor beta Human in vitro transl.
  Abnova Taiwan Corp.

Estrogen receptor beta

Estrogen receptor beta Human in vitro transl.
  Abnova Taiwan Corp.

Estrogen receptor beta

Estrogen receptor beta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Estrogen receptor beta

Estrogen receptor beta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Estrogen receptor beta (1 products)

Catalog No. Species Pres. Purity   Source  

Estrogen Receptor beta Lysate

Western Blot: Estrogen Receptor beta Lysate [NBL1-10345] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ESR2
  Novus Biologicals Inc.
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