discontinued

DM3081 Estrogen Sulfotransferase antibody

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Mouse anti Rat Estrogen Sulfotransferase EST.1

DM3081

Product Description for Estrogen Sulfotransferase

Mouse anti Rat Estrogen Sulfotransferase EST.1.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Estrogen Sulfotransferase

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Supernatant
Reactivity Rt
Applications ICC/IF, P
Clonality Monoclonal
Clone EST.1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Purified Rat Liver Estrogen Sulfotransferase.
Application Immunofluorescence. Western Blotting. Immunohistology (Formalin/paraffin): Ab. 2-4 µg/ml 30 Min. RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: Rat Liver. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Chatterjee B et. al. Chemico-Biological Interactions, 1994, 92(1-3):273-9.
  2. Demyan WF et. al. Molecular Endocrinology, 1992, 6(4):589-97.
  3. Mancini MA et. al. Endocrin
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Supernatant
Specificity
Specificity:
Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3-phosphate-5-phosphosulfate. Sulfurylated steroids do not bind to the estrogen receptor with high affinity and, therefore, are hormonally inactive. EST plays an important role in controlling the intracellular level of the receptor-active estrogens. Cellular Localization: Cytoplasmic with some Nuclear.

Mol. Wt. of Antigen: ~31kD

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