AP05110PU-N Farnesyl antibody

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0.1 mg / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Farnesyl

Product Description for Farnesyl

Rabbit anti Farnesyl.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.

Properties for Farnesyl

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, ICC/IF
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Antibody developed using Farnesyl cysteine conjugated to KLH.
Application ELISA: Detects farnesyl motif derived from isoprenylated C-A-A-X sequence. Also cross-reacts with geranylgeranyl motif. Detects KLH as well.
Background Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A 1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Clase AC, Lyman MG, del Rio T, Randall JA, Calton CM, Enquist LW, et al. The pseudorabies virus Us2 protein, a virion tegument component, is prenylated in infected cells. J Virol. 2003 Nov;77(22):12285-98. PubMed PMID: 14581565. (Free PMC Article available, 11 images available)
General Readings
  1. Baron R, Fourcade E, Lajoie-Mazenc I, Allal C, Couderc B, Barbaras R, et al. RhoB prenylation is driven by the three carboxyl-terminal amino acids of the protein: evidenced in vivo by an anti-farnesyl cysteine antibody. Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11626-31. PubMed PMID: 11027361. (Free PMC Article available, 5 images available)
  2. Lin HP, Hsu SC, Wu JC, Sheen IJ, Yan BS, Syu WJ. Localization of isoprenylated antigen of hepatitis delta virus by anti-farnesyl antibodies. J Gen Virol. 1999 Jan;80 ( Pt 1):91-6. PubMed PMID: 9934689.
  3. Aspbury RA, Prescott MC, Fisher MJ, Rees HH. Isoprenylation of polypeptides in the nematode Caenorhabditis elegans. Biochim Biophys Acta. 1998 Jun 15;1392(2-3):265-75. PubMed PMID: 9630668.
  4. Zhang FL, Casey PJ. Protein prenylation: molecular mechanisms and functional consequences. Annu Rev Biochem. 1996;65:241-69. PubMed PMID: 8811180.
  5. Gromov PS, Madsen P, Celis JE. Identification of isoprenyl modified proteins metabolically labeled with [3H]farnesyl- and [3H]geranylgeranyl-pyrophosphate. Electrophoresis. 1996 Nov;17(11):1728-33. PubMed PMID: 8982605.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Ammonium Sulfate Precipitation
Buffer System:
Phosphate buffered saline with 0.08% Sodium Azide
Liquid purified Ig fraction.
This antibody recognizes Farnesyl.

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