SM1870P Feline Coronavirus antibody

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Search for all "Feline Coronavirus"

0.25 mg / €610.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Feline Coronavirus FIPV3-70

SM1870P

Product Description for Feline Coronavirus

Mouse anti Feline Coronavirus FIPV3-70.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Feline Coronavirus

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Applications C, E, F, ICC/IF, P, WB
Clonality Monoclonal
Clone FIPV3-70
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Coronavirus cocktail.
Isotype control AM03096PU-N
Application Flow Cytometry.
Immunofluorescence.
ELISA: This antibody can be used as a Capture reagent in ELISA assays in combination with AM01094BT-N as a detection reagent.
Western Blot: Western blotting, using this antibody under reducing conditions, results in a specific band of 50-56 kDa that represents the nucleocapsid.
Immunohistochemistry on Frozen and Paraffin Sections: Microwave induced antigen retrieval in 10 mM citrate buffer is recommended prior to staining of paraffin embedded sections.
Concentration 1.0 mg/ml
General Readings
  1. Kipar A, Kremendahl J, Addie DD, Leukert W, Grant CK, Reinacher M. Fatal enteritis associated with coronavirus infection in cats. J Comp Pathol. 1998 Jul;119(1):1-14. PubMed PMID: 9717123.
  2. Kipar A, Kremendahl J, Grant CK, von Bothmer I, Reinacher M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet Pathol. 2000 Mar;37(2):129-36. PubMed PMID: 10714641.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A.
Buffer System:
PBS, pH 7.2 containing 0.05% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
This antibody reacts with Feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV).
Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.

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