NBP1-78285 FeSODB1 antibody

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Chicken anti var. FeSODB1


Product Description for FeSODB1

Chicken anti var. FeSODB1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for FeSODB1

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Aff - Purified
Reactivity var.
Applications E, WB
Clonality Polyclonal
Host Chicken
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Purified peptide from FeSODB1 protein was covalently modified to carrier protein and emulsified in adjuvant before injected in to rabbits to make anti-FeSODB1 antibodies.
Background Kinetoplastid glycosomes contain a variety of metabolic activities, such as glycolysis, beta-oxidation of fatty acids, lipid biosynthesis, and purine salvage. Confinement and sequestration of all metabolic activities tend to protect other cellular components form oxidative damages caused by oxygen radicals produced during metabolism. In Leishmaniasis, an iron superoxide dismutase (FeSODB) is expressed in low levels in the early logarithmic promastigote stage and increases toward the stationary promastigote and amastigote stages. A second FeSODB2 enzymes was cloned from the same region but its expression was high in the early logarithmic promastigote stage and decreases toward the stationary promastigote and amastigote stages. Mutations in FeSODB1 results in significant reduction in parasite in human macrophages, suggesting that FeSODB1 plays an important role in parasite growth and survival by protecting glycosomes form superoxide toxicity (1). Other parasites like trypnasoma, overexpressing mitochondrial FeSOD (iron superoxide dismutase) were more resistant to the PCD stimulus, unambiguously indicating the participation of mitochondrial O2o? in the signaling process (2). The Leishmania FeSODB1 and FeSODB2 enzymes are cloned form L chagasi promastigote cDNA library using degenerate primers derived from conserved amino acids form the MnSOD and FeSOD, the two enzymes having high homology to FeSODB1 and FeSODB2 (3). Overexpression of these FeSODs in L. chagasi parasites also showed enhanced protection against the free radical generating agents, paraquat and nitroprusside.. FeSODB1 and B2 are highly homologous proteins with a MW of 25kDa (208aa). The FeSODB1/2-selective antibodies were generated against FeSOD protein. The FeSOD1/2-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The FeSOD1 antibodies label a protein of approximately 26kDa in Western blot using Western blot positive control for FeSOD1 (PC-FeSODB1). FeSODB1/2-selective antibodies are also available in FITC conjugated preparations for confocal and immunocytochemical analyses.
Storage Store at -20C. Avoid freeze-thaw cycles.
Affinity purified
Aff - Purified

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