AM00617PU-N Fibrillin 1 / FBN1 antibody

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Mouse anti Bovine, Human Fibrillin 1 / FBN1 12A5.18

Product Description for Fibrillin 1 / FBN1

Mouse anti Bovine, Human Fibrillin 1 / FBN1 12A5.18.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Fibrillin 1 / FBN1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Bov, Hu
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone 12A5.18
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Microfibrils from zonular apparatus of bovine eye
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Cellular Localization: Extracellular microfibrils
Application Electron Microscopy.
Western Blotting (2): 1-2 µg/ml for 2 hrs at RT.
Immunohistology (Formalin/paraffin): 2-4 µg/ml for 30 min at RT; no special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Positive Control: Normal skin fibroblasts.
Background Fibrillin-1 is an extracellular acidic protein with a high cysteine content and an extended thread-like shape with mosaic composition of different types of extracellular modules. Most of the fibrillin molecule is contributed by 47 epidermal growth factor-like (EGF-like) repeats; 43 of them have a consensus sequence for calcium binding (cb). Other modules including 8-cysteine motifs (seven), hybrid motifs (two), and unique domains (three) are interspersed throughout the molecule[3-7]. fibrillin-1 contains a proline-rich region close to the N-terminus that is replaced by a glycine-rich region in fibrillin-2. The fibrillins are found throughout the connective tissue as integral components of extended fibrils.
Concentration 0.2 mg/ml
General Readings
  1. Maier A; et al. Science, 1994, 35(7):3103-10.
  2. Wright DW; et al. Matrix Biology, 1994, 14(1):41-9.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Liquid Ig fraction
This antibody detcts Fibrillin 1.
It decorates elastic framework and more specifically the oxytalantic network close to the epidermis. Cultures of normal human skin fibroblasts show discrete staining of extracellular fibrils. It shows no reaction with fibrillin 2.
Human and Cow.

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