AP21470SU-N Fibrinogen antibody

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1 ml / €260.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Mouse Fibrinogen

AP21470SU-N

Product Description for Fibrinogen

Goat anti Mouse Fibrinogen.
Presentation: Texas Red
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Fibrinogen

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms FGA, FGB, FGG
Presentation Texas Red
Reactivity Ms
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Fibrinogen is isolated from fresh plasma after removing Prothrombin.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application In precipitating techniques as Immunoelectrophoresis and Single or Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of Fibrinogen in Mouse plasma or other body fluids or to determine its concentration.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal Mouse plasma in agar-block immunodiffusion titration.
Background Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
General Readings
  1. Ploplis VA, Donahue DL, Sandoval-Cooper MJ, Caffaro MM, Sheets P, Thomas SG, Walsh M, Castellino FJ. (2014) Systemic Platelet Dysfunction is the Result of Local Dysregulated Coagulation and Platelet Activation in the Brain in a Rat Model of Isolated Traumatic Brain Injury. ahead of print. doi:10.1089/neu.2013.3302.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Labeled with Texas Red
Texas Red
State:
Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is restricted to Mouse fibrinogen molecule.
In Immunoelectrophoresis and Radial Immunodiffusion, using various antiserum concentrations against normal Mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified Fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products.
The antiserum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Mouse

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