R1152BS Fibrinogen antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Goat anti Human Fibrinogen


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  • R1152BS
  • R1152BS

Product Description for Fibrinogen

Goat anti Human Fibrinogen.
Presentation: Biotin
Product is tested for Paraffin Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Fibrinogen

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms FGA, FGB, FGG
Presentation Biotin
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Fibrinogen from Human plasma
Application Western blot: 1/500-1/2,500.
ELISA: 1/2,000-1/10,000.
This antibody has been assayed against 1.0 µg of Fibrinogen in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1/50,000 to 1/250,000 of the reconstitution concentration is suggested.
Background Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases.
The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel A. et al, and Rapold H.J. et al). All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
10-20 moles biotin per mole of IgG
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/ml BSA (immunoglobulin protease free)
Lyophilized purified Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This product detects Human Fibrinogen. Cross reactivity against Fibrinogen from other sources is unknown.
Immunoelectrophoresis give a single precipitin arc against anti-Biotin, anti-Goat serum as well as purified and partially purified Fibrinogen [Human plasma].

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