AM03212PU-S Fibroblast Surface Protein antibody

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0.5 ml / €320.00
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Mouse anti Human Fibroblast Surface Protein 1B10


Product Description for Fibroblast Surface Protein

Mouse anti Human Fibroblast Surface Protein 1B10.
Presentation: Ascites
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Fibroblast Surface Protein

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms Fibroblast Marker, Fibroblasten
Presentation Ascites
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone 1B10
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Cultured human thymic fibroblasts.
Application Immunohistochemistry on Paraffin Sections (Pretreatment of formalin fixed paraffin embedded tissue sections has not been characterized).
This product is intended for qualitative Immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissue sections, to be viewed by light microscopy. Clinical interpretation of staining results should be accompanied by histological studies with proper controls.
This antibody may be diluted to a titer of 1/25-1/50 in an ABC method for 30 min. at RT.
Positive Control: Thymus.
Background Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. It is also useful for Flow cytometry, Immunohistochemistry and Immunoblotting.
General Readings
  1. Hoyhtya, M., et al., Eur J Biochem 141(3):477-482, 1984.
  2. SunderRaj, N., et al., Curr. Eye Res. 3:637, 1984.
  3. Le, P., et al., J. Immunol., 145, 3310, 1990.
Storage Store the antibody (undiluted) at 2-8°C
Shelf life: one year from despatch.
Liquid Ascites containing Sodium Azide as preservative.
This antibody reacts with proteins of 43 kDa and 72-80 kDa molecular weight. It reacts with a Surface Protein on Fibroblasts (synovial, foreskin and thymic), fibroblast cell lines, tissue macrophages, and 95% of peripheral blood monocytes. It does not stain lymphocytes or human epithelial cells but does stain malignant fibrosarcoma tissue.
Cellular Localization: Membrane.

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