NB100-1845 Fibroblast Surface Protein antibody

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Mouse anti Human Fibroblast Surface Protein 1B10


Product Description for Fibroblast Surface Protein

Mouse anti Human Fibroblast Surface Protein 1B10.
Presentation: Ig Fraction
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for Fibroblast Surface Protein

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Fibroblast Marker, Fibroblasten
Presentation Ig Fraction
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone 1B10
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts.
Application Immunohistochemistry-Frozen, Western Blot, Optimal dilutions should be determined by the end user., Western Blot 1/500, Immunohistochemistry-Frozen
Background Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells. It is also useful for flow cytometry, immunohistochemistry and immunoblotting.
Concentration 0.2 mg/ml
General Readings
  1. Singer, K., et al., J. Invest. Dermatol., 92, 166 (1989).
  2. Hogg, N., and Horton, M., Myeloid Antigens. New and previously defined clusters, in: Leukocyte Typing III. McMichael, A., Ed., pp 576-602, Oxford University Press, Oxford (1987).
  3. SunderRaj, N., et al., Curr. Eye Res., 3, 637 (1984).
  4. Singer, K., et al., Human Immunol., 13, 161 (1985).
  5. Abbond, C., et al., Blood, 68, 1196 (1986).
  6. Le, P., et al., J. Immunol., 145, 3310 (1990).
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
0.1% Sodium Azide
Ig Fraction
The antibody recognizes an epitope on primary cultured human fibroblasts, fibroblast cell lines, tissue macrophages and 95% of peripheral blood monocytes. It does not react with vascular smooth muscle cells. The antibody may be used for removal of fibroblasts from human cultured cells by inhibition of fibroblast adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.

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