SM1214PT Fibroblasts / Epithelial Cells antibody

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Mouse anti Human Fibroblasts / Epithelial Cells D7-FIB

Product Description for Fibroblasts / Epithelial Cells

Mouse anti Human Fibroblasts / Epithelial Cells D7-FIB.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.

Properties for Fibroblasts / Epithelial Cells

Product Category Primary Antibodies
Quantity 25 µg
Synonyms Fibroblast Marker, Fibroblasten
Presentation Purified
Reactivity Hu
Applications C, F, IP
Clonality Monoclonal
Clone D7-FIB
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human foreskin Fibroblasts.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Isotype control AM03096PU-N
Application Flow Cytometry: Use 10 µl of a 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
This product is routinely tested on the KG1 cell line.
Immunohistochemistry on Frozen Tissues or Cells: 1/100.  
This antibody is reported to be sensitive to formaldehyde fixation and tissue prossesing. Use ice-cold methanol for 5 min or acetone as fixative.
Does not work on Paraffin Sections.
Background A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. J Immunol. 2007 May 1;178(9):5552-62. PubMed PMID: 17442937.
General Readings
  1. Fearns C, Dowdle EB. The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int J Cancer. 1992 Feb 20;50(4):621-7. PubMed PMID: 1537627.
  2. Kelynack KJ, Hewitson TD, Nicholls KM, Darby IA, Becker GJ. Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process. Nephrol Dial Transplant. 2000 Nov;15(11):1766-72. PubMed PMID: 11071963.
  3. van Osch GJ, van der Veen SW, Marijnissen WJ, Verhaar JA. Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage. Biochem Biophys Res Commun. 2001 Jan 26;280(3):806-12. PubMed PMID: 11162592.
  4. Behl,B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells. Biomaterials. pii: S0142-9612(13)00039-2.
  5. Morito T, Muneta T, Hara K, Ju YJ, Mochizuki T, Makino H, et al. Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans. Rheumatology (Oxford). 2008 Aug;47(8):1137-43. doi: 10.1093/rheumatology/ken114. Epub 2008 Apr 5. PubMed PMID: 18390894.
  6. Pountos I, Giannoudis PV, Jones E, English A, Churchman S, Field S, et al. NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man. J Cell Mol Med. 2011 Mar;15(3):525-34. doi: 10.1111/j.1582-4934.2010.01006.x. PubMed PMID: 20070439. (Free PMC Article available, 5 images available)
  7. Telfer JF, Brock JH. Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium. Ann Rheum Dis. 2002 Aug;61(8):741-4. PubMed PMID: 12117685. (Free PMC Article available, 2 images available)
  8. English A, Jones EA, Corscadden D, Henshaw K, Chapman T, Emery P, et al. A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis. Rheumatology (Oxford). 2007 Nov;46(11):1676-83. Epub 2007 Sep 26. PubMed PMID: 17901063.
  9. Jones EA, English A, Kinsey SE, Straszynski L, Emery P, Ponchel F, et al. Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin Cytom. 2006 Nov 15;70(6):391-9. PubMed PMID: 16977637.
  10. Nimura A, Muneta T, Koga H, Mochizuki T, Suzuki K, Makino H, et al. Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum. Arthritis Rheum. 2008 Feb;58(2):501-10. doi: 10.1002/art.23219. PubMed PMID: 18240254.
  11. Miranda-Carús ME, Balsa A, Benito-Miguel M, De Ayala CP, Martín-Mola E. Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active. Arthritis Rheum. 2004 Sep;50(9):2786-93. PubMed PMID: 15457446.
  12. Petrow PK, Wernicke D, Schulze Westhoff C, Hummel KM, Bräuer R, Kriegsmann J, et al. Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis. Ann Rheum Dis. 2002 May;61(5):391-7. PubMed PMID: 11959761. (Free PMC Article available, 6 images available)
  13. Sekiya I, Ojima M, Suzuki S, Yamaga M, Horie M, Koga H, et al. Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis. J Orthop Res. 2012 Jun;30(6):943-9. doi: 10.1002/jor.22029. Epub 2011 Dec 6. PubMed PMID: 22147634.
  14. Kanayama M, Kurotaki D, Morimoto J, Asano T, Matsui Y, Nakayama Y, et al. Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis. J Immunol. 2009 Jun 15;182(12):8015-25. doi: 10.4049/jimmunol.0900725. PubMed PMID: 19494327.
  15. Pap T, Claus A, Ohtsu S, Hummel KM, Schwartz P, Drynda S, et al. Osteoclast-independent bone resorption by fibroblast-like cells. Arthritis Res Ther. 2003;5(3):R163-73. Epub 2003 Mar 26. PubMed PMID: 12723988. (Free PMC Article available, 5 images available)
  16. Shi Y, Niedzinski JR, Samaniego A, Bogdansky S, Atkinson BL. Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration. Tissue Eng Part A. 2012 Jul;18(13-14):1313-21. doi: 10.1089/ten.TEA.2011.0357. Epub 2012 Jun 12. PubMed PMID: 22500696.
  17. Scut, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments. J Orthop Surg Res. 3: 32.
  18. Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, et al. Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum. 2002 Dec;46(12):3349-60. PubMed PMID: 12483742.
  19. Schminke, B. et al. (2014) The Pathology of Bone Tissue during Peri-Implantitis J Dent Res. pii: 0022034514559128.
  20. De Bari C, Dell'Accio F, Vanlauwe J, Eyckmans J, Khan IM, Archer CW, et al. Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis. Arthritis Rheum. 2006 Apr;54(4):1209-21. PubMed PMID: 16575900.
  21. Iyyanki, T. et al. (2015) Harvesting Technique Affects Adipose-Derived Stem Cell Yield. Aesthet Surg J. pii: sju055.
  22. Asano T, Iwasaki N, Kon S, Kanayama M, Morimoto J, Minami A, et al. α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis. Rheumatology (Oxford). 2014 Mar;53(3):415-24. doi: 10.1093/rheumatology/ket371. Epub 2013 Nov 15. PubMed PMID: 24241034.
  23. Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater. J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505. doi: 10.1002/jbm.b.33129. Epub 2014 Mar 6. PubMed PMID: 24604838. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide as preservative
Liquid purified IgG fraction from Tissue Culture Supernatant
Species Reactivity
Species reactivity (tested):
SM1214P recognizes a 112 kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS but resistant to trypsin, tunicamycin and collagenase. In immunohistochemical studies the antibody has also been found to bind to epithelium, myoepithelium, smooth muscle cells and some leucocytes.
D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (Van Osch et al., 2001). 
Negative Species: Mouse, Rat.

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